Mutation Landscape in Thyroid Cancer: Implications for Tumor Grading and Prognosis

Abstract

Background:The Aim Of The Study Was To Estimation Of The Association Between The Genetic Alterations In Proto-Oncogenes (Egfr) And Overall Thyroid Cancer.

Methods:In A Prospective Study, A Total Of Sixty (N=60) Histologically Confirmed, Previously Untreated Thyroid Cancer Patients Are In This Study. Tumour Tissue Along With Corresponding Normal Tissue Underwent An Egfr Mutation Test. The Association Of Egfr Mutation In Thyroid Cancer And Family History Were Evaluated Using Allele Specific Pcr Was Setup To Detect Mutations, If Any, In Exon 19, 20 And 21 Of Egfr.

 Results:Total Of 32 Patients 53.3% (32/60) Were Having 15bp Deletion In Exon 19 Of Egfr (P≤0.05). The Total Mutational Rate Of T790m In Egfr Tyrosine Kinase Domain (Exon 20) Among 60 Patients Was Found To Be Only 8.4% (05 Of 60) (P>0.05). The Total Of 43.3% (26 Of 60) Of Thyroid Cancer Patients Were Positive For Egfr L858r (T2573g) Mutation In Exon 21(P≤0.05).

Conclusion:A Significant Association Was Found Between Tumour Grading And T858r Mutational Status Of Egfr Gene As 34.2% Of Patients With Well Differentiated Carcinoma Were Positive For The Mutation As Opposed To 63.2% Of Patients With Poorly Differentiated Grade (P≤0.05). Significant Relations Of Multiple Variables Were Seen In Associations With Egfr Domain.