Augmented Phospholipase A2 Activity in Response to N, N- Dimethylformamide Induced Liver Toxicity in rat model

Abstract

DMF, also known as N,N-dimethylformamide, is extensively used as an organic solvent in several sectors. There are several industries that use dimethylformamide. Synthetic fibres, plastics, and other compounds are made with it, and it is also used as a solvent in industrial settings. Nevertheless, the specific function of DMF in the liver is still not well understood. Reported research indicates that different forms of Phospholipase A2 (PLA2) may contribute to liver diseases by generating powerful lipid mediators. DMF also exacerbates hepatic pathophysiology and is linked to membrane dysfunction via its impact on phospholipid metabolism. In this work, we examined the effect of DMF on the activity of Phospholipase A2 by creating rat model of acute DMF poisoning. This model was designed to simulate the effects of acute occupational poisoning in humans. Considering the complex characteristics of Phospholipase A2 enzymes, our objective was to assess the precise effects of DMF. We conducted the  measurement of phospholipase A2 activity using Thin-layer chromatography and RTPCR analysis. These methods were also used to confirm the hydrolysis of PLA2.  .