Detection of PNI using S100 protein in Oral Squamous Cell Carcinoma
DOI:
https://doi.org/10.64149/J.Carcinog.24.5s.511-515Keywords:
Oral Squamous Cell Carcinoma, Perineural Invasion, S100 immunostainAbstract
Objective: The 5th edition of the recent WHO classification of Head and Neck Tumors mentioned perineural Invasion (PNI) as one of the histopathological factors that are associated with a worse prognosis in Oral Squamous Cell Carcinoma (OSCC). The subjectivity in PNI assessment & histological simulations may contribute to underestimation of this ominous behavior of such tumors. This study was performed to detect the role of S100 as a neural marker to enhance PNI detection in OSCC.
Design: A collection of 54 formalin-fixed paraffin embedded tissue blocks of OSCC were immunostained with S100 protein to facilitate PNI identification.
Results: PNI detection was strikingly doubled upon staining with S100 protein. The number of cases showing PNI were raised from 22 to 42. Cases with hidden PNI foci or even with endoneural invasion were easily recognized upon the use of such immunostain.
Conclusion: PNI in OSCC can be easily recognized upon the use of S100 immunostain with better identification of PNI positive small nerves as well as intraneural invasion pattern.
