Characterization And Bioactivity of Nanohydroxyapatite-Gelatin Paste as A Scaffold Using Simulated Body Fluid
DOI:
https://doi.org/10.64149/J.Carcinog.24.2s.894-904Keywords:
Biomaterial, Bone regenerative therapy, Nanohydroxyapatite, Simulated body fluid, Regenerative Periodontal Treatment.Abstract
Objective: Assess the bioactivity of nanohydroxyapatite gelatin paste as a scaffold using simulated body fluid, which used to treat irregular bone defects in bone regeneration therapy and to make the bone graft placement easier during surgery.
Methods: nHA paste was prepared in this study by combining bovine-derived nHA-gelatin with hydroxypropyl methylcellulose (HPMC) at various concentrations. The nHA/G 65/35 and 60/40 pastes were immersed in SBF for 1, 2, 7, and 14 days.
Results: The highest degradation was found on day 7 in both pastes, with the most optimal swelling up to day 14, while pH revealed a significant difference except on day 2. The TEM test showed particles of nHA have an average length of 181.7 ± 20.3 nm and a diameter of 26.9 ± 1.9 nm. After immersion, FTIR demonstrated that the paste hydrolyzed as the O-H functional group and PO4 3-increased. SEM and EDS testing revealed that nHA/G 65/35 had a larger pore size and the highest Ca-P composition. Cell viability showed both pastes indicating nontoxic properties.
Conclusions: the nHA/G 65/35 paste is the best candidate for use as a scaffold due to its superior bioactivity in terms of degradability, pH, and physical, and chemical properties.
