LC-MS Profiling and Cytotoxic Evaluation of Ashwagandha and Liquorice Aqueous Extracts in HT29 and MCF-7 Cell Lines with Relevance to Chemotherapy-Induced GI Toxicity

Abstract

Chemotherapeutic agent 5FU reduces quality of life by causing many toxicity and severe GI toxicity. To evaluate this Glycyrrhiza Glabra (GG)and Withania Somnifera (WS) were examined for bioactivity in GI cell line HT 29, DPPH, and MCF 7 MTT assays. MTT studies revealed IC50 values of 36.38 ± 0.8 and 34.32 ± 0.7 µg/mL for Withania Somnifera (WS) and Glycyrrhiza Glabra (GG) for MCF-7 breast cancer cells. The invitro antioxidant experiment showed that WS had a radical scavenging activity of 134.4 ± 0.3 µg/mL, while GG had an EC50 of 99.75 ± 0.8 µg/ The proliferation assay determined GI cell line HT 29's bioactivity. WS' EC50 was 76.78 ± 0.3 µg/mL, while GG's was 77.90 ± 0.6. Studies show that WS and GG may reduce 5FU-induced GI toxicity and scavenge radicals. HPLC–ESI–MS/MS identified withanolides and Glycyrrihizic acid, demonstrating performance.  This may improve patient quality of life.