Gi50 Inhibition Study Using Sulforhodamine B (Srb) Assay Method On Mouse Melanoma Cell Line (B16-F10), Using Ethanolic Extracts Of Leaves Of Chromolaena Odorata And Capparis Moonii.

Abstract

The study investigates the in-vitro anticancer potential of ethanolic extracts from the leaves of Chromolaena odorata and Capparis moonii using the Sulforhodamine B (SRB) assay on the mouse melanoma cell line (B16-F10). These plants, rich in phytochemicals such as flavonoids and phenolic compounds, were hypothesized to possess significant anti-cancer properties. Extracts were prepared using Soxhlet apparatus and tested across multiple concentrations (10–80 μg/ml). Results demonstrated that the extract did not achieve the inhibitory concentration threshold (GI50 ≤ 20 μg/ml) defined by the National Cancer Institute for natural compounds. Morphological analysis and dose-response studies confirmed suboptimal inhibition of melanoma cell growth compared to the standard Adriamycin. While the extracts displayed a dose-dependent response, they were not effective in inhibiting melanoma growth. The findings suggest the need for enhanced experimental conditions and novel drug delivery systems to harness the therapeutic potential of these phytochemical-rich plants..