Evaluation of nootropic action of ethylacetate fraction from methanolic extract of Grewia Hirsuta bark.

Abstract

The objective of the present work was to prepare the methanolic extract of bark of Grewia hirsuta and obtain the ethyacetate fraction of the extract and evaluate its nootropic potential in rodent. The extraction was done by soxhlet apparatus and qualitative analysis of phytochemicals was done. The extraction yield of the bark of Grewia hirsuta in methanol was found to be 11.8% w/w. The fractionation of extract was achieved with ethylacetate using separating funnel. The ethyl acetate fraction was evaporated to obtain dark yellow powder in 18.2% w/w yield. The fraction tested positive for flavonoids and phenolic components. The ethylacetate fraction of methanolic extract of Grewia hirsuta was quantified for the total phenolic content. The total phenolic content of ethylacetate fraction was found to be 138.52 ± 3.511 GAE mg/g. The ethylacetate fraction of the methanolic extract of Grewia hirsuta bark was subjected to evaluation of nootropic potential using elevated plus maze test and morris water maze test at dose levels of 200 and 400 mg/kg/p.o against scopolamine induced amnesia. EAGH (200 and 400 mg/kg, p.o.) were found to be significantly improving the open arms activity (both the parameters) compared to Scopolamine treated group in the elevated plus maze test. The mice treated with 400 mg/kg EAGH required only 15.66 ± 1.211 seconds on the 4th day to find the hidden platform as compared to 51.16 ± 2.714 for the scopolamine treated mice.