Development and Validation of a UV Spectrophotometric Absorbance Correction Method for the Simultaneous Estimation of Salicylic Acid and Niacinamide in bulk and liquid dosage form

Abstract

The present study describes the development and validation of a simple, rapid, and accurate UV spectrophotometric method using the absorbance correction approach for the simultaneous estimation of salicylic acid and niacinamide in liquid dosage form. Salicylic acid and niacinamide, commonly used in dermatological formulations, exhibit overlapping UV spectra, which necessitates the use of absorbance correction for precise quantification. The method involves measuring absorbance at selected wavelengths where each drug demonstrates maximal absorption with minimal interference from the other. Validation was performed according to ICH guidelines, assessing linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), and robustness. The results demonstrated good linearity and reproducibility for both analytes within the specified concentration ranges. The proposed method is cost-effective, less time-consuming, and avoids complex sample preparation, offering a practical alternative to chromatographic techniques for routine analysis and bioanalytical studies. This method can be employed for routine   analysis in quality control laboratories.