The Role of Gut Microbiota in Metabolic and Endocrine Disorders
DOI:
https://doi.org/10.64149/J.Carcinog.24.10s.34-42Keywords:
Gut Microbiota, Metabolic Disorders, Endocrine Disorders, Obesity, Type 2 Diabetes, PCOS, 16S rRNA Sequencing, Cronbach’s Alpha, Health Awareness, Microbial CompositionAbstract
Background: The role of gut microbiota in metabolic and endocrine disorders, such as obesity, type 2 diabetes, thyroid dysfunction, and polycystic ovary syndrome (PCOS), has garnered significant attention in recent years. This study aims to explore the relationship between gut microbiota composition and these disorders, with a focus on understanding how microbial communities may contribute to disease progression.
Objectives: The primary objective of this study is to examine the gut microbiota profiles in individuals diagnosed with metabolic and endocrine disorders compared to healthy controls. Additionally, the study seeks to assess the level of awareness about gut microbiota among participants and explore potential correlations between microbial composition and health outcomes.
Methods: A cross-sectional design was used, involving 250 participants, including 125 individuals with metabolic/endocrine disorders and 125 healthy controls. Data were collected using stool samples for microbiota profiling through 16S rRNA sequencing, along with self-reported questionnaires to gather information on demographic factors, health conditions, dietary habits, and knowledge about gut microbiota. Statistical analyses, including normality tests, reliability (Cronbach’s Alpha), and correlation analyses, were performed to assess the relationship between gut microbiota composition and health indicators.
Results: The study found that age group distribution was non-normal, and Cronbach’s Alpha for the reliability of the questionnaire was low (0.16), suggesting weak internal consistency. A significant portion of participants had limited knowledge about gut microbiota, with most reporting being "somewhat familiar" or "not familiar at all" with the concept.
Conclusions: The findings suggest that gut microbiota composition may play a crucial role in metabolic and endocrine disorders. However, the study highlights the need for refinement in the measurement tools and better representation of diverse demographic groups in future research. Educational interventions to increase public awareness of gut microbiota could potentially aid in the prevention and management of these disorders.
