Shankar Sharan Singh1, Madan Lal Brahma Bhatt1, Vandana Singh Kushwaha1, Anshuman Singh2, Rajendra Kumar1, Rajeev Gupta1, Devendra Parmar2
1 Department of Radiotherapy, King George’s Medical University, Lucknow, Uttar Pradesh, India
2 Division of Developmental Toxicology, CSIR n Institute of Toxicology Research, Lucknow, Uttar Pradesh, India
DOI: 10.4103/jcar.JCar_5_16
ABSTRACT
BACKGROUND: Matrix Metalloproteinase 13 (MMP13) is a member of collagenase family and it is involved in the degradation of extracellular matrix and basement membrane protein. It is thought to be associated with tumor invasion and metastasis. Elevated MMP13 expression has been found in carcinoma of the breast, urinary bladder, head and neck and others. It is observed that MMP13 gene is also correlated with radiation response in OSCC (Oral squamous cell carcinoma) cell line based study. The present study correlates the MMP13 expressions with clinicopathological parameters and radiation response in OSCC patients. MATERIALS AND METHODS: The MMP13 mRNA levels were determined by employing qRT-PCR (real-time quantitative reverse transcriptase-polymerase chain reaction). RESULTS: We observed high expression of MMP13 mRNA in OSCC patients when compared with matched controls. Statistically significant up regulation of MMP13 mRNA expression was found in tobacco chewers, advanced T-stage (p < 0.001) and lymph node metastasis (p < 0.01). MMP13 mRNA levels were also elevated in non responders as compared to responders to radiation treatment. CONCLUSIONS: To the best of our knowledge, this is the first report that indicates role of MMP13 in radiation response in OSCC patients and could be used as potential bio-marker for radiotherapy treatment in OSCC patients.
Keywords: Matrix Metalloproteinase 13, oral squamous cell carcinoma, radiation response