Uddhav Kelavkar1, Yan Lin2, Doug Landsittel3, Uma Chandran4, Rajiv Dhir4
1Department of Urology and University of Pittsburgh Cancer Institute, 5200 Center Ave., SHMC-Suite G-37, Pittsburgh, PA, 15232, USA
2Department of Biostatistics, Graduate School of Public Health, University of Pittsburgh, 130 DeSoto Street, Pittsburgh, PA, 15261, USA
3Department of Mathematics and Computer Science, Duquesne University, Pittsburgh, PA, 15282, USA
4Department of Pathology and University of Pittsburgh Cancer Institute, 5200 Center Ave., Pittsburgh, PA, 15232, USA
DOI: 10.1186/1477-3163-5-9
ABSTRACT
One of the major components in high-fat diets (Western diet) is the omega (ω, n)-6 polyunsaturated fatty acid (PUFA) called linoleic acid (LA). Linoleic acid is the precursor for arachidonic acid (AA). These fatty acids are metabolized to an array of eicosanoids and prostaglandins depending upon the enzymes in the pathway. Aberrant expression of the catabolic enzymes such as cyclooxygenases (COX-1 and/or -2) or lipoxygenases (5-LO, 12-LO, 15-LO-1, and 15-LO-2) that convert PUFA either AA and/or LA to bioactive lipid metabolites appear to significantly contribute to the development of PCa. However, PUFA and its cellular interactions in PCa are poorly understood. We therefore examined the mRNA levels of key enzymes involved in the LA and AA pathways in 18 human donor (normal) prostates compared to 60 prostate tumors using the Affymetrix U95Av2 chips. This comparative (normal donor versus prostate cancer) study showed that: 1) the level of 15-LO-1 expression (the key enzyme in the LA pathway) is low ( P < 0.001), whereas the levels of delta-5 desaturase ( P < 0.001, the key enzyme in the AA pathway), delta-6 desaturase ( P = 0.001), elongase ( P = 0.16) and 15-lipoxygenase-2 (15-LO-2, P = 0.74) are higher in donor (normal) prostates, and 2) Contrary to the observation in the normal tissues, significantly high levels of only 15-LO-1; whereas low levels of delta-6 desaturase, elongase, delta-5 desaturase and 15-LO-2 respectively, were observed in PCa tissues. Although the cyclooxygenase (COX)-1 and COX-2 mRNA levels were high in PCa, no significant differences were observed when compared in donor tissues. Our study underscores the importance of promising dietary intervention agents such as the omega-3 fatty acids as substrate competitors of LA/AA, aimed primarily at high 15-LO-1 and COX-2 as the molecular targets in PCa initiation and/or progression.