Dezhong Joshua Liao, Yong Wang, Jiusheng Wu, Nazmi Volkan Adsay, David Grignon, Fayyaz Khanani, Fazlul H Sarkar
Department of Pathology, Wayne State University School of Medicine, And Karmanos Cancer Institute, 110 E. Warren Avenue, Detroit, MI 48201,
In order to identify good animal models for investigating therapeutic and preventive strategies for pancreatic cancer, we analyzed pancreatic lesions from several transgenic models and made a series of novel findings. Female MT- tgfα mice of the MT100 line developed pancreatic proliferation, acinar-ductal metaplasia, multilocular cystic neoplasms, ductal adenocarcinomas and prominent fibrosis, while the lesions in males were less severe. MT- tgfα-ES transgenic lines of both sexes developed slowly progressing lesions that were similar to what was seen in MT100 males. In both MT100 and MT- tgfα-ES lines, TGFα transgene was expressed mainly in proliferating ductal cells. Ela- myc transgenic mice with a mixed C57BL/6, SJL and FVB genetic background developed pancreatic tumors at 2-7 months of age, and half of the tumors were ductal adenocarcinomas, similar to what was reported originally by Sandgren et al . However, in 20% of the mice, the tumors metastasized to the liver. MT100/Ela- myc and MT- tgfα-ES/Ela- myc double transgenic mice developed not only acinar carcinomas and mixed carcinomas as previously reported but also various ductal-originated lesions, including multilocular cystic neoplasms and ductal adenocarcinomas. The double transgenic tumors were more malignant and metastasized to the liver at a higher frequency (33%) compared with the Ela- myc tumors. Sequencing of the coding region of p16ink4 , k- ras and Rb cDNA in small numbers of pancreatic tumors did not identify mutations. The short latency for tumor development, the variety of tumor morphology and the liver metastases seen in Ela- myc and MT- tgfα/Ela- myc mice make these animals good models for investigating new therapeutic and preventive strategies for pancreatic cancer.