Design, Synthesis and Mechanistic Perspectives of Novel Coumarin Derivatives in Cancer Therapy

Abstract

Coumarins, a class of naturally occurring benzopyrone derivatives, have emerged as promising scaffolds in the development of novel anticancer therapeutics. Their diverse biological activities are attributed to the structural flexibility of the coumarin nucleus, which allows extensive functionalization at different positions of the aromatic ring. This study reports the rational design and synthesis of novel coumarin derivatives and evaluates their mechanistic potential in cancer therapy. A series of substituted coumarin analogs were synthesized via Pechmann condensation and subsequent functional modifications, including halogenation, nitration, and Schiff base formation. The compounds were characterized by FTIR, NMR, and LC-MS analyses. In silico molecular docking studies revealed significant interactions of selected derivatives with key oncogenic targets, including topoisomerase II, tubulin, and VEGFR-2. In vitro cytotoxicity assays (MTT method) against MCF-7, HeLa, and A549 cancer cell lines demonstrated promising activity of halogenated and Schiff base–coupled coumarins with IC₅₀ values in the micromolar range. Mechanistic insights indicated apoptosis induction via caspase activation, cell cycle arrest at G2/M phase, and suppression of angiogenesis-related pathways. Collectively, these findings highlight the therapeutic potential of coumarin scaffolds in anticancer drug development and pave the way for future clinical translation.