Hassanain Jwad Abidalhussein 1, Wejdan Ridha Taj-Aldeen 2
1Dentistry Collage, Karbala University, Kerbala, IRAQ
2Collage of Science, Babylon University, Babile, IRAQ
ABSTRACT
With the progress of science, interest has become in linking modern science with the nano-aspect and the application of nanotechnology in all fields of biological science and medicine, among the most important of which is the biosynthesis of nanoparticles and their application as antimicrobials. In our current research, iron nanoparticles (Fe NPs) were synthesized by using oyster shell as a raw material. oyster shell converted into powder after purification, drying and grinding, and immersing it in de-ionized water in order to be ready for responding through its mixture with hydrochloric acid, as a first step for this synthesis process. The adding of sodium hydroxide is the step with the continuation of the process to extract the iron nanoparticles (Fe NPs) from the oyster shell powder through using a hot plate magnetic stirrer devise (1000 rpm, 50 C). The standard properties of iron nanoparticles were confirmed by using X-ray diffraction (XRD), scanning electron microscopy (SEM), and (FTIR)- based analysis, they were applied as an antibacterial agent that inhibits the action of bacteria. Objective: The objective of our research is to apply the biosynthesize of iron nanoparticles (Fe NPs) as an antimicrobial agent through a serious of steps, starting by the oyster shells grinding within standard specifications that allow them to perform their role as antibiotics. Methods: The working methods includes the collection of oyster shells, then they transformed to powder. The extract of iron nanoparticles (Fe NPs) from oyster shells includes a set of steps interspersed with the break the bonds that bind the basic components of the oyster shell components by using HCL, and NaOH solution. Results and Characterization: The results performed using the (SPSS) statistical program, and the particle sizes of iron nanoparticles (Fe NPs) are calculated by using XRD crystallite (grain) calculator (Scherrer Equation). The characterization of (Fe NPs) are performed by using (X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and FTIR-Based analysis), it gave particles of acceptable size ranging between (30-90) nanometers. Conclusion: We conclude that the biosynthesis of nanoparticles is the successful way to obtain and apply them as an antimicrobial, instead of using antibiotics that are made from chemicals in unsafe and environmentally friendly ways
Keywords:Green biosynthesis, Iron nanoparticles, Antimicrobial agent.