Mohammad-Hassan Khadem-Ansari1, Zahra Shahsavari1, Yousef Rasmi1, Rahim Mahmoodlo2
1Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
2Department of Surgery, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
DOI: 10.4103/1477-3163.79683
ABSTRACT
Aims:To measure oxidative DNA and lipid damages, urinary levels of 8-hydroxy-2Ͳ-deoxyguanosine (8-OHdG), and 8-isoprostane in esophageal squamous cell carcinoma (SCC) patients and compare the values with that in controls. Materials and Methods: The urinary concentrations of 8-OHdG and 8-isoprostane were measured in 32 SCC patients (13 female/19 male; mean age: 61.4 ± 10.5 years) and 45 controls (22 female/23 male; mean age: 58.1 ± 8.3 years). Results: Squamous cell carcinoma patients showed significantly higher levels of urinary 8-OHdG (15.6 ± 5.1 ng/mg creatinine) than controls (5.8 ± 2.1 ng/mg creatinine) (P<.001). Increased urinary concentrations of 8-isoprostane were also detected in SCC patients (35.4 ± 6.5 ng/mmol creatinine) as compared to the controls (16.9 ± 4.0 ng/mmol creatinine) (P<.001). Conclusions: Our results show the presence of oxidative DNA and lipid damage in the SCC patients. This may have a connection to carcinogenesis in the esophagus.
Keywords: Esophageal squamous cell carcinoma, 8-hydroxy-2′-deoxyguanosine, 8-isoprostane